Description

The Mouse Phylogeny Viewer (MPV) is a custom genome browser designed to provide the user with reliable and detailed answers to questions on the haplotype diversity and phylogenetic origin of the genetic variation underlying any genomic region of most laboratory strains (both classical and wild-derived). A detailed explanation of the methods used and meaning of the data has been reported in "Subspecific origin and haplotype diversity in the laboratory mouse" (Yang et al. 2011).

MPV allows the user to select a region of the genome and a set of laboratory strains and/or wild caught mice (i.e, samples). The region is selected by specifying the start (e.g. 31200000 or 31200K or 31.2M), and end of the interval and the chromosome (i.e, autosome number and X chromosome). Samples can be selected by name or by entire set.

Data sets

SNPs on the Mouse Diversity Array

This display provides local density of SNPs on the Mouse Diversity Array. The browser provides a histogram in which the height of the bar is proportional to the local SNP density.

Subspecific Origin

In windows representing large genomic regions, this section depicts each sample as a solid line.

Blue bars represent M. m. domesticus haplotypes
Red lines represent M. m. musculus haplotypes
Green lines represent M. m. castaneus haplotypes
White lines represent regions of haplotypes of undetermined origin
Purple lines represent heterozygous regions with M. m. domesticus and M. m. musculus haplotypes
Cyan lines represent heterozygous regions with M. m. domesticus and M. m. castaneus haplotypes
Brown lines represent heterozygous regions with M. m. musculus and M. m. castaneus haplotypes

After zooming to a smaller region, each sample is depicted by the same horizontal line described above and a variable number of vertical bars. These bars denote the presence of diagnostic alleles in that sample at that marker (SNP or VINO). The height of each bar represents the diagnostic score for that allele while the color represents the subspecies (M. m. domesticus (blue), M. m. musculus (red) and M. m. castaneus (green)).

Heterozygosity Regions

Each sample is depicted as a line. White represents homozygous regions and black bars represent heterozygous regions. The height of the vertical bars represents the local level of heterozygosity.

For classical inbred strains, MPV provides access to four additional types of analysis.

Compatibility Intervals

These are overlapping intervals that show no evidence of historical recombination. The browser provides a histogram representing the local density of intervals. Under this track there are two additional tracks in which each interval is shown as a blue bar. The user needs to zoom in to regions with a high density of intervals to be able to see individual intervals. For each such compatible interval there is a phylogenetic tree, in which each node corresponds to a haplotype and each edge to SNPs with the same strain distribution pattern (see below).

IBD

The browser identifies regions of identity by descent among all selected classical strains for a given region. IBD regions are shown as pink bars.

Haplotype Coloring

This section depicts each sample as a colored line using eight pastel colors. The order of the samples can be changed at will by the user. Samples can be recolored based on the strain order in the display.

Phylogeny Trees

Trees are available for each compatible interval by clicking on that interval. The browser provides the distance tree, the distance matrix and the strains belonging to each haplotype. Selected strains are shown in bold. Colors represent subspecific origin. For each haplotype we provide a score and the number of SNP used to support it. Asterisks, denote haplotypes for which IBD among all samples is not strongly supported (high score and/or low number of SNPs). The user can take into consideration the trees in neighboring intervals to weight the evidence supporting a given haplotype. Phylogenetic trees including wild-derived PWK, WSB, and CAST can be seen in the CCV (csbio.unc.edu/ccv)

Further details are explained in our paper (see reference).

Viewer

Strain and subset selection

Filter

Chromosome

Build 37

Start

End

3000000 = 3,000,000 = 3m = 3M = 3000k = 3KK

Gene Lookup

Start typing a gene name

Type a strain name to search. Multiple strains can be selected.
Click the box next to the set name to select all.

View filter data

Navigation

10x 2x 2x 10x

To zoom in, click and drag across a track to highlight a region, then click [Zoom]

Tracks

Reference

Jeremy R. Wang, Fernando Pardo-Manuel de Villena, and Leonard McMillan. Comparative analysis and visualization of multiple collinear genomes. BMC Bioinformatics, 2012.